Elsevier

Gene Reports

Volume 26, March 2022, 101497
Gene Reports

Investigation of CYP2E1 and Caspase-3 Gene Expressions in COVID-19 patients

https://doi.org/10.1016/j.genrep.2022.101497Get rights and content

Highlights

  • Downregulation of CYP2E1 gene expression in critic Covid-19 patients

  • Increased Caspase-3 mRNA expression in Covid-19 patients

  • There is no correlation between caspase-3 and CYP2E1 gene expression in covid-19 patients.

  • Several laboratory finding have been related with CYP2E1 gene expression.

Abstract

Background

COVID-19 pandemic spread around the world like an infectious disease that presents waved effects on patients. Some patients needed ICU and respiratory support. Some patients only had flu-like symptoms. Cytokine storm and elevated ROS were serious problems for treatment. Apoptotic genes and CYP Family are part of these mechanisms.

Aim

In this study, our aim was to examine the gene expression CYP2E1 and Caspase-3 in patients with COVID-19 infection.

Method

60 COVID-19(+) patients (ICU and non-ICU patients) and 30 healthy volunteers were enrolled to study. To measure the level of gene expression qPCR was used. The 2-ΔΔCt method was utilized to analyze gene expression.

Results

The expression of CYP2E1 and Caspase-3 genes showed a significant discrepancy between patients and healthy individuals. Caspase-3 expression increased (p=0,0041) but CYP2E1 expression decreased (p=0,0214) in COVID-19 patients compared to healthy individuals. Both levels of gene expression were lower in patients with affected lungs than patients with unaffected lungs (p<0,05). Laboratory findings including d-Dimer, LDH, platelet count, lymphocyte count were related to both gene expressions (p<0,05). We found no correlation between CYP2E1 and Caspase-3 expressions.

Conclusion

The expression of Caspase-3 demonstrated apoptotic situations of patients but was not related to the CYP2E1 expression level. CYP2E1 gene expression is an important actor to metabolize endogens and xenobiotics however, COVID-19 patients demonstrated decreased CYP2E1 expression. CYP2E1 and Caspase-3 gene expression levels may be used as a diagnostic tool for COVID-19 patients.

Keywords

CCYP2E1
COVID-19
Caspase-3
SARS-CoV-2

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