Issue 79, 2021

Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor

Abstract

We developed a one-minute, one-step SARS-CoV-2 antigen assay based on protein-induced fluorescence enhancement of a DNA aptamer. The system showed significant selectivity and sensitivity towards both nucleocapsid protein and SARS-CoV-2 virus lysate, but with marked improvements in speed and manufacturability. We hence propose this platform as a mix-and-read testing strategy for SARS-CoV-2 that can be applied to POC diagnostics in clinical settings, especially in low- and middle-income countries.

Graphical abstract: Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor

Supplementary files

Article information

Article type
Communication
Submitted
27 Jul 2021
Accepted
06 Sep 2021
First published
15 Sep 2021
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2021,57, 10222-10225

Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor

J. M. Lee, C. R. Kim, S. Kim, J. Min, M. Lee and S. Lee, Chem. Commun., 2021, 57, 10222 DOI: 10.1039/D1CC04066A

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