Yonsei Med J. 2020 Sep;61(9):826-830. English.
Published online Aug 27, 2020.
© Copyright: Yonsei University College of Medicine 2020
Brief Communication

Use of Darunavir-Cobicistat as a Treatment Option for Critically Ill Patients with SARS-CoV-2 Infection

Eun Jin Kim,1,* Sun Ha Choi,2,* Jae Seok Park,3 Yong Shik Kwon,3 Jaehee Lee,4 Yeonjae Kim,5 Shin Yup Lee,2 and Eun Young Choi6
    • 1Division of Pulmonary and Critical Care Medicine, Daegu Catholic University Medical Center, Daegu, Korea.
    • 2Division of Pulmonary and Critical Care Medicine, Kyungpook National University Chilgok Hospital, Daegu, Korea.
    • 3Division of Pulmonology, Department of Internal Medicine, Keimyung University Dongsan Hospital, Keimyung University School of Medicine, Daegu, Korea.
    • 4Department of Internal Medicine, School of Medicine, Kyungpook National University, Daegu, Korea.
    • 5Division of Pulmonary and Critical Care Medicine, Daegu Fatima Hospital, Daegu, Korea.
    • 6Division of Pulmonary and Critical Care Medicine, Yeungnam University Medical Center, Daegu, Korea.
Received May 21, 2020; Revised July 11, 2020; Accepted July 28, 2020.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We retrospectively reviewed patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections who were admitted to an intensive care unit in Daegu, South Korea. The outcomes of patients who did (cases) or did not (controls) receive darunavir-cobicistat (800–150 mg) therapy were compared. Fourteen patients received darunavir-cobicistat treatment, and 96 received other antiviral therapy (controls). Overall, the darunavir-cobicistat group comprised patients with milder illness, and the crude mortality rate of all patients in the darunavir-cobicistat group was lower than that in the controls [odds ratio (OR) 0.20, 95% confidence interval (CI) 0.04–0.89, p=0.035]. After 1:2 propensity-score matching, there were 14 patients in the darunavir-cobicistat group, and 28 patients in the controls. In propensity score-matched analysis, the darunavir-cobicistat group had lower mortality than the controls (OR 0.07, 95% CI 0.01–0.52, p=0.009). In conclusion, darunavir-cobicistat therapy was found to be associated with a significant survival benefit in critically ill patients with SARS-CoV-2 infection.

Keywords
Coronavirus disease 2019; severe acute respiratory syndrome coronavirus 2; darunavir-cobicistat

On March 11, 2020, the World Health Organization (WHO) declared the coronavirus disease 2019 (COVID-19) a pandemic. Until May 20, 2020, there were more than 4.9 million reported COVID-19 cases and 324869 deaths across more than 200 countries. Currently, there are no specific therapeutic agents for treating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Potential drugs for treating COVID-19 include human immunodeficiency virus (HIV) type 1 aspartate protease inhibitors, such as lopinavir and darunavir, which have been shown to inhibit SARS-CoV in vitro, the cause of SARS in humans.1, 2, 3, 4 Cobicistat-boosted darunavir is a boosted protease inhibitor in a fixed-dose combination that is approved for use in treating HIV type 1 infection.5, 6 Drug efficacy evaluation in cell models in vitro have revealed that darunavir is active against SARS-CoV-2.2 At present, however, there are no clinical data on the use of these drugs for COVID-19.

Cobicistat-boosted darunavir is stable as a suspension,7 so it was considered to be suitable for administration as a nasogastric tube to critical ill patients. Here, we evaluated the effects of darunavir-cobicistat on the clinical outcomes of critically ill patients with COVID-19 using a risk stratification model that adjusted for potential differences between the darunavir-cobicistat treated and non-darunavir-cobicistat treated individuals.

We retrospectively reviewed the medical records of all adults with laboratory-confirmed SARS-CoV-2 infection who were subsequently admitted to an intensive care unit (ICU) at one of the seven tertiary or referral hospitals in Daegu, South Korea between February 18 and April 5, 2020. A WHO guidance document defines laboratory confirmation of SARS-CoV-2 infection as a positive result of a real-time reverse transcriptase-polymerase chain reaction assay of samples obtained from nasal and pharyngeal swabs. The first part of this study was a retrospective cohort study that included all patients who were critically ill with laboratory-confirmed SARS-CoV-2 infection. Cobicistat-boosted darunavir was assigned to patients who failed other antiviral treatments or at the physician's discretion. The outcomes of patients who did (cases) or did not (controls) receive darunavir-cobicistat (800–150 mg) therapy were compared. The second part of the investigation comprised a matched (1:2) case-control study, with the patients who did or did not receive darunavir-cobicistat therapy designated as “cases” and “controls” (Fig. 1), respectively. This study was conducted in accordance with the tenets of the Declaration of Helsinki and was reviewed and approved by the Institutional Review Board at each hospital. The requirement for informed consent was waived because of the retrospective study design (IRB Number: YUH IRB 2020-03-057, IRB Institution: Yeungnam University Medical Center).

Fig. 1
Schematic representation of the data analysis plan for the cohort study and the matched case-control study. *Adjusted aged ≥65 years, Sequential Organ Failure Assessment. OR, odds ratio; CI, confidence interval.

The present study included 110 critically ill patients who received intensive care for COVID-19, of whom 14 received darunavir-cobicistat treatment and 96 received other antiviral therapy.

There were no significant intergroup differences with regard to age, sex ratio, body mass index, and underlying disease/conditions between the groups who did and did not receive darunavir-cobicistat treatment (Table 1). Sequential Organ Failure Assessment (SOFA) scores {median score 2 [interquartile range (IQR) 2–4] vs. 6 [IQR 3–8], p<0.001} and National Early Warning Scores [median score 6 (IQR 5–7) vs. 8 (IQR 5–11), p<0.001] were significantly lower in the darunavir-cobicistat group than in the controls, indicating that the darunavir-cobicistat group had patients with milder illness. The incidence of shock and acute respiratory distress syndrome (ARDS) during hospitalization did not differ between the two groups. There were no between-group differences in the length of ICU stay or survival, although the number of deaths was significantly lower in the darunavir-cobicistat treatment group (14.3% vs. 46.9%, p=0.021). There were also significant differences in the numbers of patients who survived and were discharged from the ICU: 10 (83.3%) in the darunavir-cobicistat group and 29 (39.2%) in the controls (p=0.004). The crude mortality rates of all patients and ARDS patients in the darunavir-cobicistat group were lower than those in the controls [odds ratio (OR) 0.20, 95% confidence interval (CI) 0.04–0.89, p=0.035 and OR 0.17, 95% CI 0.04–0.79, p=0.024, respectively) (Table 2). Mortality rates after adjusting for variables, such as age ≥65 years and SOFA score, were the same in both groups. Two variables, age ≥65 years and SOFA score, were selected for propensity-score matching based on outcomes of the univariate and multivariate analyses. After 1:2 propensity-score matching, there were 14 patients in the darunavir-cobicistat group and 28 in the controls. Table 1 shows the patient characteristics for both groups after matching the propensity scores; the two groups were balanced. The number of deaths was significantly lower in the darunavir-cobicistat group (14.3% vs. 50.0%, p=0.025). In the propensity score-matched analysis, the darunavir-cobicistat group had lower mortality than the controls (OR 0.09, 95% CI 0.01–0.52, p=0.009) (Table 2). Furthermore, the darunavir-cobicistat group, which comprised ARDS patients, had lower mortality than the controls (OR 0.08, 95% CI 0.01–0.50, p=0.008). To determine the effect of darunavir-cobicistat on COVID-19, we subjected the matched cohorts to Kaplan-Meier analysis and noted a significant between-group difference in in-hospital survival rate (p=0.048, respectively) (Fig. 2).

Fig. 2
Kaplan-Meier analysis for survival outcomes with darunavir-cobicistat (DRV-COVI) therapy plotted against time after admission with p values by log-rank test.

Table 1
Baseline Demographics and Clinical Characteristics (n=110) of the Study Population

To our knowledge, this is the first report to evaluate the treatment effect of darunavir-cobicistat in critically ill COVID-19 patients in whom darunavir-cobicistat therapy was associated with a significant survival benefit over standard treatment.

The mechanism of action underlying the effect of darunavir-cobicistat on SARS-CoV-2 remains to be elucidated. Several studies have evaluated the therapeutic effects of antiretroviral drugs in COVID-19, although the results are controversial. A recent randomised clinical trial by Cao, et al.8 investigated the efficacy of lopinavir-ritonavir (400–100 mg, twice daily for 14 days) treatment in COVID-19 and showed no beneficial effect thereof over standard care in hospitalised adults with severe COVID-19. Regarding antiviral therapy with darunavir-cobicistat, there are no human clinical data on the use of this drug combination in COVID-19 patients, although a randomised clinical trial of darunavir-cobicistat is ongoing in China (NCT04252274).

This study has several limitations. First, because the study was retrospective, the possibility of selection bias cannot be excluded. Second, the propensity score matching method is limited because it adjusted only for observed covariates. Finally, there was a selection bias of using cobicistat-boosted darunavir only in 14 patients with physician preference and interference with other antiviral agents. Owing to this limitation, the conclusions of nonrandomised studies that use propensity scores must be assessed for consistency with other results, as well as for biological plausibility. Therefore, we suggest that preclinical studies and prospective clinical studies are needed to confirm these preliminary results.

In conclusion, darunavir-cobicistat therapy was associated with a significant survival benefit in critically ill patients with SARS-CoV-2 infection in this study. Further study of darunavir-cobicistat in relation to this highly virulent disease is needed.

Notes

The authors have no potential conflicts of interest to disclose.

AUTHOR CONTRIBUTIONS:

  • Conceptualization: Eun Young Choi.

  • Data curation: all authors.

  • Formal analysis: Eun Jin Kim and Sun Ha Choi.

  • Funding acquisition: Eun Young Choi.

  • Investigation: all authors.

  • Methodology: Eun Jin Kim, Sun Ha Choi, and Eun Young Choi.

  • Software: Eun Jin Kim, Sun Ha Choi, and Eun Young Choi.

  • Validation: Eun Jin Kim, Sun Ha Choi, and Eun Young Choi.

  • Writing—original draft: Eun Jin Kim, Sun Ha Choi, and Eun Young Choi.

  • Writing—review & editing: Sun Ha Choi and Eun Young Choi.

  • Approval of final manuscript: all authors.

ACKNOWLEDGEMENTS

This work was supported by the Research Program of Medicity Daegu Council funded by Daegu Metropolitan City (fund code COVID19-DM18).

References

    1. Chu CM, Cheng VC, Hung IF, Wong MM, Chan KH, Chan KS, et al. Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings. Thorax 2004;59:252–256.
    1. China News Network. Abidol and darunavir can effectively inhibit coronavirus [accessed on 2020 February 5].
    1. Dong L, Hu S, Gao J. Discovering drugs to treat coronavirus disease 2019 (COVID-19). Drug Discov Ther 2020;14:58–60.
    1. Sanders JM, Monogue ML, Jodlowski TZ, Cutrell JB. Pharmacologic treatments for coronavirus disease 2019 (COVID-19): a review. JAMA. 2020 Aug 13;
    1. Curran A, Pérez-Valero I, Moltó J. Rezolsta® (darunavir/cobicistat): first boosted protease inhibitor co-formulated with cobicistat. AIDS Rev 2015;17:114–120.
    1. Putcharoen O, Do T, Avihingsanon A, Ruxrungtham K. Rationale and clinical utility of the darunavir-cobicistat combination in the treatment of HIV/AIDS. Drug Des Devel Ther 2015;9:5763–5769.
    1. Zanon D, Manca A, De Nicolò A, D'Avolio A, Musazzi UM, Cilurzo F, et al. Data on the stability of darunavir/cobicistat suspension after tablet manipulation. Data Brief 2020;30:105552
    1. Cao B, Wang Y, Wen D, Liu W, Wang J, Fan G, et al. A trial of lopinavir-ritonavir in adults hospitalized with severe Covid-19. N Engl J Med 2020;382:1787–1799.

Metrics
Share
Figures

1 / 2

Tables

1 / 2

Funding Information
PERMALINK