Journal of Biological Chemistry
Volume 298, Issue 4, April 2022, 101739
Methods and ResourcesImproved SARS-CoV-2 main protease high-throughput screening assay using a 5-carboxyfluorescein substrate
Under a Creative Commons license
open access
Keywords
high-throughput screening
FRET
viral protease
protease substrate
SARS-CoV-2
main protease (Mpro)
3CL protease (3CLpro)
enzyme kinetics
coronavirus
Abbreviations
AMC
7-amino-4-methylcoumarin
COVID-19
coronavirus disease 2019
DABCYL
4-(4-dimethylaminophenylazo)benzoic acid
DLS
dynamic light scattering
DMSO
dimethyl sulfoxide
DNP
2,4-dinitrophenyl
EDANS
5-(2-aminoethylamino)-1-naphthalenesulfonic acid
FAM
5-carboxyfluorescein
HCoV
human coronavirus
HTS
high-throughput screen
MCA
7-methoxycoumarin-4-acetic acid
MERS-CoV
Middle East respiratory syndrome coronavirus
Mpro
main protease
nanoDSF
nano-differential scanning fluorimetry
PDB
Protein Data Bank
PLpro
papain-like protease
pp1a
polyprotein 1a
pp1ab
polyprotein 1ab
SARS-CoV-2
severe acute respiratory syndrome coronavirus 2
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© 2022 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology.