NYC Metropolitan Wastewater Reveals Links between Sars-Cov-2 Amino Acid Mutations and Disease Outcomes

28 Pages Posted: 3 Mar 2023

See all articles by Archana Anand

Archana Anand

San Francisco State University

Chenghua Long

Columbia University

Kartik Chandran

Columbia University

Abstract

Since late 2020, diverse SARS-CoV-2 variants with enhanced infectivity and transmissibility have emerged. In contrast to the focus on amino acid mutations in the spike protein, mutations in non-spike proteins and their associated impacts remain relatively understudied. New York City metropolitan wastewater revealed over 60% of the most frequently occurring amino acid mutations in regions outside the spike protein. Strikingly, ~50% of the mutations detected herein remain uncharacterized for functional impacts.  Our results suggest that there are relatively understudied mutations within non-spike proteins N, ORF1a, ORF1b, ORF9b, and ORF9c, that increase transmissibility, and infectivity among human populations. We also demonstrate significant correlations of P314L, D614G, T95I, G50E, G50R, G204R, R203K, G662S, P10S, and P13L with documented mortality rates, hospitalization rates, and percent positivity.

Keywords: wastewater, amino acid, SARS-CoV-2, mortality, infectivity, variant

Suggested Citation

Anand, Archana and Long, Chenghua and Chandran, Kartik, NYC Metropolitan Wastewater Reveals Links between Sars-Cov-2 Amino Acid Mutations and Disease Outcomes. Available at SSRN: https://ssrn.com/abstract=4377336 or http://dx.doi.org/10.2139/ssrn.4377336

Archana Anand (Contact Author)

San Francisco State University ( email )

1600 Holloway Avenue
San Francisco, CA 94132
United States

Chenghua Long

Columbia University ( email )

3022 Broadway
New York, NY 10027
United States

Kartik Chandran

Columbia University ( email )

3022 Broadway
New York, NY 10027
United States

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