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Identification of Specific Biomarkers for Selective Clinical Interventions in Post- COVID-19 Patients

54 Pages Posted: 5 Oct 2022

See all articles by Debora Paris

Debora Paris

National Research Council (CNR) - Institute of Biomolecular Chemistry

Letizia Palomba

University of Urbino Carlo Bo - Department of Biomolecular Sciences

Maria Cristina Albertini

University of Urbino Carlo Bo - Department of Biomolecular Sciences

Annabella Tramice

National Research Council (CNR) - Institute of Biomolecular Chemistry

Lorenzo Motta

Ospedale Santa Maria della Misericordia

Eleonora Giammattei

University of Urbino Carlo Bo - Department of Biomolecular Sciences

Pasquale Ambrosino

Istituti Clinici Scientifici Maugeri IRCCS - Cardiac Rehabilitation Unit

Andrea Motta

National Research Council (CNR) - Institute of Biomolecular Chemistry

Mauro Maniscalco

Istituti Clinici Scientifici Maugeri IRCCS - Pulmonary Rehabilitation Unit

More...

Abstract

Background: In COVID-19 clinical symptoms can persist even after negativization. Currently, there are no specific diagnostic tests that characterize the complexity of post COVID-19 and address specific, interventions. We here investigated the biomarkers that define the post-COVID-19 clinical state.

Methods: We investigated the exhaled breath condensate (EBC) of 38 post COVID-19 patients and 38 sex and age-matched healthy controls via nuclear magnetic resonance (NMR)-based metabolomics. Predicted gene-modulated microRNAs (miRNAs) related to COVID-19 were quantified from EBC of 6 patients and 6 controls. Finally, clinical parameters from all post-COVID19 patients were correlated with metabolomic data.

Findings: Post-COVID-19 patients and controls showed different metabolic phenotype ('metabotype'), due to persistent inflammation. From them, by using enrichment analysis, we identified miR146a, miR-126 and miR-223, which resulted up-regulated (miR146a) and downregulated (miR-126 and miR-223) in post-COVID-19. Correlation of metabolites with clinical data confirmed that patients and controls presented different metabotypes, and that post-COVID-19 before rehabilitation is still characterized by persistent inflammation, dysregulation of liver, endovascular thrombotic and pulmonary processes, and physical impairment.

Interpretation: Joining EBC metabolomics, miRNAs and clinical data, we described the altered condition of post-COVID-19 patients. They are characterized by several pathophysiological processes, which should be primarily targeted to contrast the post-acute sequelae of COVID-19.

Funding Information: This work was partially supported by the “Ricerca corrente” funding scheme of the Ministry of Health, Italy.

Declaration of Interests: All the authors wish to declare no conflict of interest.

Ethics Approval Statement: We followed the STROBE reporting guidelines, 7 in line with the 1975 Declaration of Helsinki. The Ethic Committee of Istituto Nazionale Tumori, Fondazione Pascale, Naples, Italy approved the study (n. ICS 3/20). All study participants provided written informed consent.

Keywords: SARS-CoV-2, COVID-19, metabolomics, biomarkers, microRNAs, disability, rehabilitation, outcomes

Suggested Citation

Paris, Debora and Palomba, Letizia and Albertini, Maria Cristina and Tramice, Annabella and Motta, Lorenzo and Giammattei, Eleonora and Ambrosino, Pasquale and Motta, Andrea and Maniscalco, Mauro, Identification of Specific Biomarkers for Selective Clinical Interventions in Post- COVID-19 Patients. Available at SSRN: https://ssrn.com/abstract=4238640 or http://dx.doi.org/10.2139/ssrn.4238640

Debora Paris

National Research Council (CNR) - Institute of Biomolecular Chemistry ( email )

Letizia Palomba

University of Urbino Carlo Bo - Department of Biomolecular Sciences ( email )

Maria Cristina Albertini

University of Urbino Carlo Bo - Department of Biomolecular Sciences ( email )

Annabella Tramice

National Research Council (CNR) - Institute of Biomolecular Chemistry ( email )

Lorenzo Motta

Ospedale Santa Maria della Misericordia ( email )

Eleonora Giammattei

University of Urbino Carlo Bo - Department of Biomolecular Sciences ( email )

Pasquale Ambrosino

Istituti Clinici Scientifici Maugeri IRCCS - Cardiac Rehabilitation Unit ( email )

Andrea Motta (Contact Author)

National Research Council (CNR) - Institute of Biomolecular Chemistry ( email )

Mauro Maniscalco

Istituti Clinici Scientifici Maugeri IRCCS - Pulmonary Rehabilitation Unit ( email )

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