Evaluation of Loop-Mediated Isothermal Amplification for Detecting COVID-19

12 Pages Posted: 27 Nov 2022

See all articles by Astuti Giantini

Astuti Giantini

University of Indonesia (UI) - Universitas Indonesia Hospital

Muhammad Suhaeri

University of Indonesia (UI) - Universitas Indonesia Hospital

Ardiana Kusumaningrum

University of Indonesia (UI) - Universitas Indonesia Hospital

Dimas Seto Prasetyo

University of Indonesia (UI) - Universitas Indonesia Hospital

Windy Sahar

University of Indonesia (UI) - Universitas Indonesia Hospital

Rakhmad Hidayat

University of Indonesia (UI)

Muhammad Arza Putra

University of Indonesia (UI) - Universitas Indonesia Hospital

Hermawan Hermawan

University of Indonesia (UI) - Universitas Indonesia Hospital

Abstract

The emergence of SARS-CoV-2 has caused worldwide pandemic of COVID-19. Infection is difficult to diagnose early as some patients remain asymptomatic and may carry this virus to other people. Currently, qRT-PCR is the widely accepted mode for detection. However, the need for sophisticated instrument and trained personnel may hinder its application, especially in remote and facility-lacking areas. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) may serve as a potential approach for detection of SARS-CoV-2 as the resources needed in its application is far less complex than those of qRT-PCR. Herein, we evaluated RT-LAMP based analytical method (COVIDNow), relative to qRT-PCR, in detecting SARS-CoV-2 by using 63 clinical respiratory samples. Based on our finding, COVIDNow exhibited sensitivity and specificity values of 87.5% and 80.6%, respectively. Taken together, RT-LAMP based detection of SARS-CoV-2 by utilizing COVIDNow might serves as a valuable diagnostic tool in the management of global COVID-19 pandemic condition.

Note:
Funding Information: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Conflict of Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Ethical Approval: The sample collection, use, and data analysis were approved by the Ethic Committee of Universitas Indonesia Hospital (001/SKPE/KKO/2020/00) and subjects gave their consent prior to participation.

Keywords: Covid-19, RT-LAMP, SARS-CoV-2, Diagnosis, qRT-PCR

Suggested Citation

Giantini, Astuti and Suhaeri, Muhammad and Kusumaningrum, Ardiana and Prasetyo, Dimas Seto and Sahar, Windy and Hidayat, Rakhmad and Putra, Muhammad Arza and Hermawan, Hermawan, Evaluation of Loop-Mediated Isothermal Amplification for Detecting COVID-19. Available at SSRN: https://ssrn.com/abstract=4258934 or http://dx.doi.org/10.2139/ssrn.4258934

Astuti Giantini

University of Indonesia (UI) - Universitas Indonesia Hospital ( email )

Muhammad Suhaeri

University of Indonesia (UI) - Universitas Indonesia Hospital ( email )

Depok, 16424
Indonesia

Ardiana Kusumaningrum

University of Indonesia (UI) - Universitas Indonesia Hospital ( email )

Dimas Seto Prasetyo (Contact Author)

University of Indonesia (UI) - Universitas Indonesia Hospital ( email )

Windy Sahar

University of Indonesia (UI) - Universitas Indonesia Hospital ( email )

Depok, 16424
Indonesia

Rakhmad Hidayat

University of Indonesia (UI) ( email )

Muhammad Arza Putra

University of Indonesia (UI) - Universitas Indonesia Hospital ( email )

Depok, 16424
Indonesia

Hermawan Hermawan

University of Indonesia (UI) - Universitas Indonesia Hospital ( email )

Depok, 16424
Indonesia

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