Feline Coronavirus Detection in Clinically Suspected Feline Infectious Peritonitis Cats
8 Pages Posted: 22 Dec 2021 Publication Status: Preprint
Abstract
Feline infectious peritonitis (FIP) is a fatal immune-mediated disease in cat caused by feline coronavirus (FCoV). Feline coronavirus infections were widespread reported throughout the world. However the evidence and confirm data regarding its presence is rarely reported in Indonesia. This study aims to investigate the FCoV local isolates and provide its present genetic information. Total 95 clinically suspected FIP patients were included in this study. Specimen samples collected were effusion, blood, feces, or tissue. Molecular assay was conducted using nested RT-PCR which targeted 3-‘UTR and partial S gene. The sequence genome was analyzed using software MEGA-X. FCoV was detected in 72 patients with 89% was type I FCoV. High positivity rate was obtained in cats with effusion (78.7%), male 82%), and young age (75.3%). FCoV local isolates were clearly separated into two main clusters in which cluster divided into several groups. Based on partial S gene detection, type I FCoV was detected more frequent. Gender showed significant association to the disease. Even though, molecular detection cannot be the solely tool to obtain definitive diagnosis FIP, it strongly supported the diagnosis. Molecular detection is also useful for early detection in shelter, breeder, or multiple household to prevent fatal disease. This study provided the first genetic analysis of FCoV in Indonesia. Furthermore, complete genome sequence is needed to determine detail genetic pattern of Indonesia local isolates.
Keywords: diagnosis, feline coronavirus, feline infectious peritonitis, molecular detection, RT-PCR
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