Vol. 14 No. 1 (2022): Archives of Public Health
Public Health

Polymerase chain reaction test for detection of SARS-CoV-2 virus and variants

Aneta Peshnachka
Institute of Public Health of the Republic of North Macedonia, Skopje, Republic of North Macedonia
Golubinka Boshevska
Institute of Public Health of the Republic of North Macedonia, Skopje, Republic of North Macedonia
Nevenka Velichkova
Goce Delchev University, Faculty of Medical Sciences, Stip, Republic of North Macedonia
Elizabeta Jancheska
Institute of Public Health of the Republic of North Macedonia, Skopje, Republic of North Macedonia
Maja Vukovikj
Institute of Public Health of the Republic of North Macedonia, Skopje, Republic of North Macedonia
Teodora Buzharova
Institute of Public Health of the Republic of North Macedonia, Skopje, Republic of North Macedonia
Gordana Nikolovska
Institute of Public Health of the Republic of North Macedonia, Skopje, Republic of North Macedonia
Ardian Preshova
Institute of Public Health of the Republic of North Macedonia, Skopje, Republic of North Macedonia
Shaban Memeti
Institute of Public Health of the Republic of North Macedonia; Ss Cyril and Methodius University in Skopje, Faculty of Medicine, Republic of North Macedonia

Published 2022-06-23

Keywords

  • SARS-CoV-2,
  • RT-PCR,
  • variant of Concern - VOC,
  • variant of interest - VOI

How to Cite

1.
Peshnachka A, Boshevska G, Velichkova N, Jancheska E, Vukovikj M, Buzharova T, Nikolovska G, Preshova A, Memeti S. Polymerase chain reaction test for detection of SARS-CoV-2 virus and variants. Arch Pub Health [Internet]. 2022 Jun. 23 [cited 2024 Mar. 29];14(1):1-11. Available from: https://www.id-press.eu/aph/article/view/6030

Abstract

The first human cases of coronavirus disease (COVID-19), a disease caused by the new coronavirus, was reported on December 31, 2019 in China for the first time. Тhe virus was named temporarily as 2019 new coronavirus (2019 novel coronavirus-2019 -nCoV), or  finally as SARS-CoV-2. The aim of this paper is to give an overview of the laboratory detection of SARS-CoV-2 virus with reverse transcriptase - polymerase chain reaction (RT-PCR) in real time, as well as detection of viral mutations in the Laboratory for virology at the Institute of Public Health. The samples used in the study were taken from all geographical regions in North Macedonia, including the 10 regional Centers for Public Health throughout the country, from hospital patients, from COVID19 hospitals across the country and from the COVID19 checkpoint at the Institute of Public Health. All samples were tested using RT-PCR in real time. Additional assays were applied for identification of the circulating variants. The continuous surveillance of the variants of concern (VOC), as well as the newly emerged variants can allow the public health officials to modify their approach to disease control and management and intervene more effectively as well as in a timely manner in order to prevent major morbidity and mortality from COVID-19.

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References

  1. "WHO Director-General's opening remarks at the media briefing on COVID-19—11 March 2020". World Health Organization. Retrieved 11 March 2020
  2. Yong SK, Su PC, Yang YS. Molecular Targets for the Testing of COVID-19. Biotechnol J. 2020 Jun;15(6):e2000152. DOI: https://doi.org/10.1002/biot.202000152
  3. WHO/COVID-19 Weekly Epidemiological Update, Edition 42, published 1 June 2021
  4. Работно упатство за начин на земање примероци од лица суспектни за КОВИД -19. Институт за јавно здравје достапно на: http://iph.mk
  5. http://iph.mk/postapka-za-transport-na-primeroci-za-laboratoriska-analiza-za-sars-cov-2-covid-19-od-bolnici-centri-za-javno-zdravje-i-sobirni-punktovi/
  6. Guidance on regulations for the transport of infectious substances 2019 – 2020: applicable from 1 January 2019. Available at: https://apps.who.int/iris/handle/10665/325884
  7. QIAGEN®, QIAamp® Viral RNA Mini Handbook. Available at: https://www.qiagen.com › resources
  8. Maccura Biotechnology Co., Ltd, Mag-Bind RNA Extraction Kit. Available at: https://www.maccura.com/en/product
  9. Viral DNA and RNA Extraction kit (Plate). Available at: https://aitbiotech.com/abGenix
  10. SaMag-96 Nucleic Acids Purification System Available at: https://sacace.com/public_ftp/sacace-samag-96.zip
  11. Thermo Scientific™- MagMAX™ Viral/Pathogen II Nucleic Acid, Isolation Kit достапно на: https://www.thermofisher.com/order/catalog/product/A48383#/A48383
  12. Xpert® Xpress SARS-CoV-2. Available at https://www.cepheid.com/en_US/tests/Critical-Infectious-Diseases/Xpert-Xpress-SARS-CoV-2
  13. Allplex 2019-nCoV Assay. Available at: https://www.seegene.com/assays/allplex_2019_ncov_assay#
  14. BGI’s Real-Time Fluorescent RT-PCR kit Available at: https://www.bgi.com/global/molecular-genetics/2019-ncov-detection-kit/
  15. Sansure Biotech. Available at:
  16. http://www.sansureglobal.com/product/novel-coronavirus2019-ncov-nucleic-acid-diagnostic-kit-pcr-fluorescence-probing-2/
  17. Charite- Diagnostic detection of Wuhan coronavirus 2019 by real-time RT-PCR Available at:https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf
  18. RealTime SARS-CoV-2. Available at: https://www.euroimmun.com/products/molecular-genetic-diagnostics/pd/molecular-infection-diagnostics/sars-cov-2/2606/8/104696/
  19. TaqMan 2019-nCoV Assay Kit v1 Available at: https://www.thermofisher.com/order/catalog/product/A47532#/A47532
  20. SARS-CoV-2 Fluorescent PCR. Available at: https://www.fda.gov/media/137026/download, SARS-CoV-2 Fluorescent PCR Kit, Maccura
  21. TaqPath™ COVID-19 Combo Kit, Multiplex real-time RT-PCR test intended for the qualitative detection of nucleic acid from SARS CoV 2. Available at: thermofisher.com
  22. SARS-CoV-2/Influenza Multiplex. Available at: www.dna-technology.ru/SARS-CoV2
  23. Genrui SARS-CoV-2 Detection Kit. Available at: http://www.genrui-bio.com/products-detail.php?ProId=74
  24. WHO/Europe & ECDC Methods for the detection and identification of SARS-CoV-2 variants March. 2021; Available at: https://www.euro.who.int/en/health-topics/health-emergencies/coronavirus-covid-19/publications-and-technical-guidance/2021/methods-for-the-detection-and-identification-of-sars-cov-2-variants,-march-2021
  25. SARS-CoV-2 Variant Plus RT-qPCR. Available at: https://www.bioeksen.com.tr/en/biospeedy--sars cov2-variant-plus
  26. VirSNiP SARS-CoV-2 Spike N501Y Available at: https://www.tib-molbiol.de/covid-19#nav-644
  27. WHO. Laboratory biosafety guidance related to coronavirus disease (COVID-19), Interim guidance. 2020, Available at https://www.who.int/publications/i/item/laboratory-biosafety-guidance-related-to-coronavirus-disease-(covid-19)
  28. World Health Organization. Diagnostic testing for SARS-CoV-2: interim guidance. 2020. Available at: https://apps.who.int/iris/handle/10665/334254. License: CC BY-NC-SA 3.0 IGO
  29. Guidance for surveillance of SARS-CoV-2 variants Available at: https://www.who.int/publications/i/item/WHO_2019-nCoV_surveillance_variants