Sample-Pooling Strategy for SARS-CoV-2 Detection among Students and Staff of the University of Sannio
Abstract
:1. Introduction
2. Materials and Methods
2.1. Informed Consent
2.2. Swab Collection and Preparation
2.3. SARS-CoV-2 RT-qPCR
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
SARS-CoV-2 | Severe Acute Respiratory Syndrome Coronavirus 2 |
WHO | World Health Organization |
RT-qPCR | Reverse Transcription-Quantitative Polymerase Chain Reaction |
IVD | For In Vitro Diagnostic use |
IHD | In-House developed |
COVID-19 | Coronavirus Disease of 2019 |
RP | RNAseP gene |
RdRP | RNA-dependent RNA Polymerase gene |
RdRP/Hel | RNA-dependent RNA Polymerase/Helicase gene |
N | Nucleocapsid Protein gene |
E | Envelope gene |
BSL-2 | Biosafety Level 2 |
CDC | Center for Disease Control and Prevention |
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Primer/Probe Name | Sequence (5′→3′) |
---|---|
2019-nCoV_N1-P | [FAM/VIC]ACCCCGCATTACGTTTGGTGGACC[BHQ1] |
2019-nCoV_N1-F | GACCCCAAAATCAGCGAAA |
2019-nCoV_N1-R | TCTGGTTACTGCCAGTTGAATCTG |
hRP-Probe | [HEX]TTCTGACCTGAAGGCTCTGCGCG[BHQ1] |
hRP-F | AGATTTGGACCTGCGAGCG |
hRP-R | GAGCGGCTGTCTCCACAAGT |
RdRP_SARSr-P2 | [CY5]CAGGTGGAACCTCATCAGGAGATGC[BBQ650] |
RdRP_SARSr-F2 | GTGARATGGTCATGTGTGGCGG |
RdRP_SARSr-R1 | CARATGTTAAASACACTATTAGCATA |
Samples | CT Values | Positivity to SARS-CoV-2 | ||||||
---|---|---|---|---|---|---|---|---|
N IVD | N IHD | N IHD POOL | RdRP/HEL IVD | RdRP IHD | RdRP IHD POOL | E IVD | ||
UNI_2409_276 | 25.93 | 25.87 | 27.96 | 26.61 | 38.37 | 34.35 | N/A | Confirmed |
UNI_2809_015 | 30.06 | 24.39 | 20.06 | 27.95 | 32.70 | 30.45 | 30.94 | Confirmed |
UNI_2809_147 | N/A | 33.76 | 28.77 | N/A | 30.15 | 34.82 | N/A | Not confirmed |
UNI_2809_178 | 24.89 | 19.36 | 19.67 | 26.59 | 24.21 | 23.02 | N/A | Confirmed |
UNI_3009_230 | 25.33 | 24.05 | 22.74 | 28.17 | 30.33 | 44.44 | 17.13 | Confirmed |
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Polvere, I.; Silvestri, E.; Sabatino, L.; Giacco, A.; Iervolino, S.; Peluso, T.; Guida, R.; Zerillo, L.; Varricchio, R.; D’Andrea, S.; et al. Sample-Pooling Strategy for SARS-CoV-2 Detection among Students and Staff of the University of Sannio. Diagnostics 2021, 11, 1166. https://doi.org/10.3390/diagnostics11071166
Polvere I, Silvestri E, Sabatino L, Giacco A, Iervolino S, Peluso T, Guida R, Zerillo L, Varricchio R, D’Andrea S, et al. Sample-Pooling Strategy for SARS-CoV-2 Detection among Students and Staff of the University of Sannio. Diagnostics. 2021; 11(7):1166. https://doi.org/10.3390/diagnostics11071166
Chicago/Turabian StylePolvere, Immacolata, Elena Silvestri, Lina Sabatino, Antonia Giacco, Stefania Iervolino, Teresa Peluso, Rosa Guida, Lucrezia Zerillo, Romualdo Varricchio, Silvia D’Andrea, and et al. 2021. "Sample-Pooling Strategy for SARS-CoV-2 Detection among Students and Staff of the University of Sannio" Diagnostics 11, no. 7: 1166. https://doi.org/10.3390/diagnostics11071166