Antispike Immunoglobulin-G (IgG) Titer Response of SARS-CoV-2 mRNA-Vaccine (BNT162b2): A Monitoring Study on Healthcare Workers
Abstract
:1. Introduction
- Elderly 65 years of age or older: people aged 65 and over should receive a booster injection. The risk of severe COVID-19 disease increases with age.
- Long-term care facility residents aged 18 and over: Long-term care facility residents live closely together in group settings.
- People with comorbidities between the ages of 18 and 64.
- People who work or live in high-risk environments between the ages of 18 and 64.
2. Materials and Methods
2.1. General Characteristics
- -
- dental area: dental physicians, chair assistants, hygienists, and nurses: a total of 90 were evaluated. (39.13% of 230 total)
- -
- radiological area: radiology physicians, technicians, and nurses: a total of 72 were evaluated (31.30% of a total of 230)
- -
- internal medicine area: a total of 34 were evaluated (14.78% of a total of 230);
- -
- Forensic Medicine area: a total of 34 (14.78% of 230 total) were evaluated.
- Group I: subjects between 20–30 years old;
- Group II: subjects between 30–40 years old;
- Group III: subjects between 40–50 years old;
- Group IV: subjects between 50–60 years old;
- Group V: subjects between 60–70 years old;
2.2. Statistical Analysis
3. Results
- Between the age of 20 and 30 years old there were 45 subjects (19.57% of 230 enrolled).
- Between the age of 30 and 40 years old there were 52 subjects (22.61% of 230 enrolled)
- Between the age of 40 and 50 years old there were 34 subjects (14.78% of 230 enrolled).
- Between the age 50 and 60 years old there were 53 subjects (23.04% of 230 enrolled)
- Between the age of 60 and 70 years of age there were 46 subjects (20% of 230 enrolled).
3.1. Statistical Findings
3.1.1. Age-Related Findings
3.1.2. Blood-Type-Related Findings
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
ACE2 | angiotensin-converting enzyme-2 |
ACE | angiotensin-converting enzyme |
ACE1 | angiotensin-converting enzyme 1 |
AIFA | Agenzia Italiana del Farmaco |
Alfa | English variant B.1.1.7 |
anti-RBD IgG | Immunogloublin G anti receptor-binding domain |
Antispike | Test IgG Antispike |
BAU | unità arbitrarie vincolanti |
Beta variant | (former of South Africa) |
BMI | Body mass index |
CI | Interval of confidence |
CLIAs | chemiluminescence immunoassay |
CRP | C-reactive protein |
Delta | Indian variant B.1.617.2 |
ELISA | enzyme-linked immunosorbent assay |
EMA | European Medicines Agency |
ETA | variant B.1.525; Date of designation March 2021 |
Gamma | Brasilian variant P.1 |
hACE2 receptor | human angiotensin I-converting enzyme 2 receptor |
IFN | Interferon |
IgA | Immunoglobulins A |
IgG | Immunoglobulins G |
IgM | Immunoglobulins M |
IOTA | variant B.1.526; earliest documented samples USA (November 2020), Date of designation March 2021 |
IQR | Interquartile range |
KAPPA | Indian variant B.1.617.1 |
LAMBA | variant C.37; earliest documented samples Peru (August 2020), Date of designation June 2021 |
LFIAs | lateral flow immunoassays. |
MERS | Middle East Respiratory Syndrome |
MMF | mycophenolate mofetil |
MPA | mycophenolic acid |
MPPDH | inosine-5’-monophosphate dehydrogenase |
NAAT | nucleic acid amplification test |
NGS | Next Generation Sequencing |
bNAbs | Broadly neutralizing antibodies |
N-IgG | Anti-N-IgG |
PRD | Viral Prion-like domain |
RBD | receptor-binding domain |
RBDs | receptor-binding domains |
RDB-IgG | receptor-binding domain neutralizing antibodies |
RT-PCR | real-time PCR Polymerase chain reaction |
S | the Spike glycoprotein |
SARS-CoV-1 | Severe Acute Respiratory Syndrome Coronavirus 1 |
SARS-CoV-2 | Severe Acute Respiratory Syndrome Coronavirus 2 (COVID-19) |
SARSr-CoV Rp3 | salivar protein similar to fused 8a and 8b SARS-CoV Beta Coronavirus |
S-IgG | Antispike IgG |
thio-NAD | thionicotinamide-adenine dinucleotide |
TNF | Tumor Necrosis Factor |
VIPIT | prothrombotic immune thrombocytopenia |
VOC | Variants of Concern |
VOI | Variants of Interest |
ZETA | variant P.2 |
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All Blood Types | |||
---|---|---|---|
Titer 1 | Titer 2 | Titer 3 | |
Average | 8.413 | 3.880 | 1.473 |
St.Dev | 9.510 | 5.156 | 1.818 |
Max | 64.771 | 44.352 | 15.455 |
Min | 882 | 103 | 77 |
Range: | 63.889 | 44.249 | 15.377 |
# Patients | 229 | ||
Titer 1,2 | Titer 2,3 | Titer 1–3 | |
Correlation | 0.97 | 0.80 | 0.81 |
Ages Related Blood Types | |||
---|---|---|---|
Titer 1 | Titer 2 | Titer 3 | |
Average | 8.413 | 3.880 | 1.473 |
St.Dev | 9.510 | 5.156 | 1.818 |
Max | 64.771 | 44.352 | 15.455 |
Min | 882 | 103 | 77 |
Range: | 63.889 | 44.249 | 15.377 |
# Patients | 229 | ||
Titer 1,2 | Titer 2,3 | Titer 1–3 | |
Correlation | 0.97 | 0.80 | 0.81 |
Genders Referred Blood Types | |||
---|---|---|---|
Titer 1 | Titer 2 | Titer 3 | |
Average | 8.413 | 3.880 | 1.473 |
St.Dev | 9.510 | 5.156 | 1.818 |
Max | 64.771 | 44.352 | 15.455 |
Min | 882 | 103 | 77 |
Range: | 63.889 | 44.249 | 15.377 |
# Patients | 229 | ||
Titer 1,2 | Titer 2,3 | Titer 1–3 | |
Correlation | 0.97 | 0.80 | 0.81 |
Group I 20–30 yo | Group II 31–40 yo | Group III 41–50 yo | Group IV 51–60 yo | Group V 61–70 yo | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
titer 1 | titer 2 | titer 3 | titer 1 | titer 2 | titer 3 | titer 1 | titer 2 | titer 3 | titer 1 | titer 2 | titer 3 | titer 1 | titer 2 | titer 3 | |
Mean | 6342 | 2207 | 207.6 | 5118 | 1628 | 151.3 | 3871 | 1172 | 169.0 | 4046 | 1963 | 1010 | 6438 | 2289 | 780.6 |
SD | 5506 | 2397 | 599.8 | 6593 | 2041 | 377.0 | 4737 | 1522 | 414.3 | 4174 | 3872 | 3413 | 10673 | 5513 | 2578 |
Lower 95% CI | 4668 | 1479 | 25.29 | 3264 | 1054 | 45.30 | 2243 | 657.3 | 28.83 | 2896 | 895.2 | 69.02 | 3269 | 670.2 | 23.75 |
Upper 95% CI | 8016 | 2936 | 390.0 | 6972 | 2202 | 257.4 | 5498 | 1687 | 309.2 | 5197 | 3030 | 1951 | 9608 | 3907 | 1537 |
0/+ | 0/− | |||||
---|---|---|---|---|---|---|
Titer 1 | Titer 2 | Titer 3 | Titer 1 | Titer 2 | Titer 3 | |
Mean | 10,289 | 5025 | 1739 | 16,810 | 8710 | 3561 |
SD | 10,013 | 6024 | 1548 | 15,992 | 9160 | 4414 |
Lower 95% CI of mean | 8001 | 3648 | 1385 | 27.84 | 903 | −1071 |
Upper 95% CI | 12,577 | 6401 | 2093 | 33,593 | 18,322 | 8192 |
A/+ | A/− | |||||
Titer 1 | Titer 1 | Titer 1 | Titer 1 | Titer 2 | Titer 3 | |
Mean | 7327 | 5717 | 5717 | 5717 | 8710 | 3561 |
SD | 8160 | 3095 | 3095 | 3095 | 9160 | 4414 |
Lower 95% CI of mean | 5008 | 3638 | 3638 | 3638 | 903 | −1071 |
Upper 95% CI | 9646 | 7797 | 7797 | 7797 | 18,322 | 8192 |
B/+ | B/− | |||||
Titer 1 | Titer 2 | Titer 1 | Titer 2 | Titer 1 | Titer 2 | |
Mean | 5867 | 2574 | 5867 | 2574 | 5867 | 2574 |
SD | 7293 | 2965 | 7293 | 2965 | 7293 | 2965 |
Lower 95% CI of mean | 2856 | 1350 | 2856 | 1350 | 2856 | 1350 |
Upper 95% CI | 8877 | 3798 | 8877 | 3798 | 8877 | 3798 |
AB/+ | ||||||
Titer 1 | Titer 1 | Titer 1 | ||||
Mean | 4945 | 4945 | 4945 | |||
SD | 3577 | 3577 | 3577 | |||
Lower 95% CI of mean | 2672 | 2672 | 2672 | |||
Upper 95% CI | 7218 | 7218 | 7218 |
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Inchingolo, A.D.; Malcangi, G.; Ceci, S.; Patano, A.; Corriero, A.; Azzollini, D.; Marinelli, G.; Coloccia, G.; Piras, F.; Barile, G.; et al. Antispike Immunoglobulin-G (IgG) Titer Response of SARS-CoV-2 mRNA-Vaccine (BNT162b2): A Monitoring Study on Healthcare Workers. Biomedicines 2022, 10, 2402. https://doi.org/10.3390/biomedicines10102402
Inchingolo AD, Malcangi G, Ceci S, Patano A, Corriero A, Azzollini D, Marinelli G, Coloccia G, Piras F, Barile G, et al. Antispike Immunoglobulin-G (IgG) Titer Response of SARS-CoV-2 mRNA-Vaccine (BNT162b2): A Monitoring Study on Healthcare Workers. Biomedicines. 2022; 10(10):2402. https://doi.org/10.3390/biomedicines10102402
Chicago/Turabian StyleInchingolo, Alessio Danilo, Giuseppina Malcangi, Sabino Ceci, Assunta Patano, Alberto Corriero, Daniela Azzollini, Grazia Marinelli, Giovanni Coloccia, Fabio Piras, Giuseppe Barile, and et al. 2022. "Antispike Immunoglobulin-G (IgG) Titer Response of SARS-CoV-2 mRNA-Vaccine (BNT162b2): A Monitoring Study on Healthcare Workers" Biomedicines 10, no. 10: 2402. https://doi.org/10.3390/biomedicines10102402