Detection of SARS-CoV-2 by using real-time PCR nasopharyngeal swabs in suspected patients and their clinical medication

https://doi.org/10.1016/j.sintl.2021.100148Get rights and content
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Highlights

  • Molecular detection of SARS-CoV-2.

  • REAL TIME PCR nasopharyngeal swabs detection.

  • Medication use for treatment for covid-19.

  • Covid-19 relation with clinical test: Lactate dehydrogenase, serum ferritin level, D-Dimer, TG, cholesterol, thyphidot, HDL, lymphocyte count and CRP.

Abstract

Background

The corona name derived from their crown like spike proteins attach with cell receptors. It belongs to coronaviradae family and nideovirales order, envelop virus, size range 65–125 ​nm and positive single standard RNA between 26.4 and 31.7 ​kb and contain 7096 amino acid. There are four subtypes that have been detected these are alpha, beta, gamma and delta.

Methodology

The 267 covid-19 blood and nasopharyngeal samples were collected from Multan region. RNA extraction from nasopharyngeal samples and run the PCR. The blood samples use for clinical tests, Lactate dehydrogenase, serum ferritin level, D-Dimer, TG, cholesterol, thyphoidot, HDL, lymphocyte count and CRP.

Results

127 (47.21%) out of 267 patients were covid-19 PCR positive and showed the amplification of ORF1ab, E, and N gene, while 140 individuals were covid-19 PCR negative and not showed the amplification of ORF1ab, E and N gene. The patients with negative Covid-19 PCR, the other analysis tests such as lactate dehydrogenase, HDL, ferritin, ESR, CBP, D-Dimer, Tg, cholesterol, CRP and CT scan. The patients effected covid-19 have higher values of D-Dimer, ESR, Neutrophils, LDH, CRP and ferritin level than normal ranges. However, the values of HDL, cholesterol and lymphocytes were decreased from the normal range.

Keywords

SARS-CoV-2
Covid-19
CRP (c reactive protein)
LDH (Lactate dehydrogenase)
HDL (High density lipid)
TG (Triglyceride)
ESR (Erythrocyte sedimentation rate)
CBP (Complete blood picture)

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