Objectives The aim of this study was to investigate the association between disagreed group chat and perceived stress among medical residents in university hospital. Methods The participants were 62 medical residents in Chosun university hospital. Stress were evaluated by the Perceived Stress Scale. Pearson correlation was carried out to examine the association between numbers of group chat room and total scores of stress scale. In addition, χ2 test was performed to compare frequency of each perceived stress scale with or without disagreed chat rooms in study participants. Results There is no significant correlation between numbers of disagreed group chat and total scores of stress scale. However, for the 10th perceived stress subscale, “You feel calm”, the frequency of those who checked the low score in the group with the disagreed group chat was significantly higher (p-value = 0.030). In addition, for the 27th perceived stress subscale, “You have trouble relaxing”, the frequency of those who checked the high score in the group with the disagreed group chat was significantly higher (p-value = 0.037). Conclusion This study demonstrates the perceived stress frequency is higher in the group with disagreed group chat room than in the other group without. Our findings suggest that using one or more disagreed group chat room might be stressful for medical residents.