Sars-Cov-2 Monitoring in Municipal Wastewaters of Three Canadian Cities with Different Population Sizes as Driven by the Delta and Omicron Variants and Their Sub-Lineages

26 Pages Posted: 18 Apr 2022

See all articles by Femi Francis Oloye

Femi Francis Oloye

affiliation not provided to SSRN

YUWEI XIE

University of Saskatchewan

Mohsen Asadi

University of Saskatchewan

Jenna Cantin

affiliation not provided to SSRN

Jonathan K. Challis

University of Saskatchewan

Markus Brinkmann

University of Saskatchewan - School of Environment and Sustainability, Toxicology Centre; University of Saskatchewan - Global Institute for Water Security

Kerry McPhedran

University of Saskatchewan

Kevin Kristian

affiliation not provided to SSRN

Mark Keller

affiliation not provided to SSRN

Mike Sadowski

affiliation not provided to SSRN

Paul D. Jones

University of Saskatchewan

Chrystal Landgraff

Public Health Agency of Canada

Chand Mangat

Public Health Agency of Canada

Meghan Fuzzen

University of Waterloo

Mark R. Servos

University of Waterloo

John Giesy

University of Saskatchewan

Abstract

Highlights• Viral load of SARS-CoV-2 RNA was measured in three wastewater treatment plants serving communities with different population sizes• Whole-genome sequencing of RNA from wastewater revealed AY.12, AY.25, AY.27, AY.93 as common AY-lineages• Omicron became the dominant VOC within a short time in the three cities• Traces of Omicron-sub-lineage BA.2 were detected through sequencing at the sub-consensus level.

Keywords: wastewater, Variant of Concerns, SARS-COV-2 RNA, Chemical tracer, BA.1, BA.2

Suggested Citation

Oloye, Femi Francis and XIE, YUWEI and Asadi, Mohsen and Cantin, Jenna and Challis, Jonathan K. and Brinkmann, Markus and McPhedran, Kerry and Kristian, Kevin and Keller, Mark and Sadowski, Mike and Jones, Paul D. and Landgraff, Chrystal and Mangat, Chand and Fuzzen, Meghan and Servos, Mark R. and Giesy, John, Sars-Cov-2 Monitoring in Municipal Wastewaters of Three Canadian Cities with Different Population Sizes as Driven by the Delta and Omicron Variants and Their Sub-Lineages. Available at SSRN: https://ssrn.com/abstract=4086335 or http://dx.doi.org/10.2139/ssrn.4086335

Femi Francis Oloye (Contact Author)

affiliation not provided to SSRN ( email )

No Address Available

YUWEI XIE

University of Saskatchewan ( email )

Mohsen Asadi

University of Saskatchewan ( email )

College of Education
Saskatoon, S7N 5A7
Canada

Jenna Cantin

affiliation not provided to SSRN ( email )

No Address Available

Jonathan K. Challis

University of Saskatchewan ( email )

Markus Brinkmann

University of Saskatchewan - School of Environment and Sustainability, Toxicology Centre ( email )

University of Saskatchewan - Global Institute for Water Security ( email )

Kerry McPhedran

University of Saskatchewan ( email )

College of Education
Saskatoon, S7N 5A7
Canada

Kevin Kristian

affiliation not provided to SSRN ( email )

No Address Available

Mark Keller

affiliation not provided to SSRN ( email )

No Address Available

Mike Sadowski

affiliation not provided to SSRN ( email )

No Address Available

Paul D. Jones

University of Saskatchewan ( email )

Chrystal Landgraff

Public Health Agency of Canada ( email )

Canada

Chand Mangat

Public Health Agency of Canada ( email )

Canada

Meghan Fuzzen

University of Waterloo ( email )

Waterloo, N2L 3G1
Canada

Mark R. Servos

University of Waterloo ( email )

John Giesy

University of Saskatchewan ( email )

College of Education
Saskatoon, S7N 5A7
Canada

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