COVID-19C5aR inhibition of nonimmune cells suppresses inflammation and maintains epithelial integrity in SARS-CoV-2–infected primary human airway epithelia
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Key words
SARS-CoV-2
complement
anaphylatoxin receptors
primary human airway epithelial cells
Abbreviations used
3D
3-Dimensional
ALI
Air-liquid interphase
ARDS
Acute respiratory distress syndrome
BF
Brightfield
C3
Complement component 3
C3aR/C5aR
C3a/C5a receptor, anaphylatoxin receptors
COVID-19
Coronavirus disease 2019
CPE
Cytopathic effect
dpi
Day postinfection
HAE
Human airway epithelia
IC
Intracellular
MAC
Membrane attack complex
MCP-1
Monocyte chemotactic protein 1
MOI
Multiplicity of infection
PFU
Plaque-forming unit
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
TCC
Terminal complement complex
TEER
Transepithelial electrical resistance
UI
Uninfected
Cited by (0)
The authors were supported by the Austrian Science Fund (FWF; grant no. P 34070-B to W.P. and grant no. P33510-B to D.W.), Marie Skłodowska-Curie action (CORVOS EU-H2020-MSCA-ITN-2019; grant no. 860064 to D.W.), the Anniversary Fund of the Austrian National Bank (OeNB; grant no. P 17614 to W.P. and grant no. 17633 to D.W.), and the National Institute of Allergy and Infectious Diseases (grant no. UM1AI068618 to D.W.).
Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest.
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These authors contributed equally to this work.
© 2021 The Authors. Published by Elsevier Inc. on behalf of the American Academy of Allergy, Asthma & Immunology.