Coronaviruses are a relatively large (125 nm), diverse group of RNA viruses infecting many mammals, amphibians, reptiles, and birds^1,2. Coronavirus contains a positive-sense RNA (+ RNA) genome that directly acts as messenger RNA (mRNA) during viral replication inside the host cell³. Coronaviruses are spherical particles with a viral envelope, which is acquired from host cytoplasmic membrane⁴. Although coronaviruses are enveloped, the envelope is very sensitive to temperature, chemicals, disinfectants, soaps, and sanitizers⁵. The viral envelope is crucial for host cell attachment, leading to further replication steps⁶. If the viral envelope is disintegrated, coronaviruses cannot proceed with replication⁷. On the viral envelope surface, there are spike glycoproteins, which are very important for attachment to host cell receptors⁸. Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) viral spike glycoproteins attach to angiotensin-converting enzyme-2 (ACE-2) receptors on pneumocytes⁹. ACE-2 receptors are located in the lungs, heart, arteries, kidneys, and intestines¹⁰. Recent case studies have revealed that many patients with coronavirus disease-2019 (COVID-19) have also died of myocardial infarction, stroke, and renal failure^11–13. This suggests that after viremia, SARS-CoV-2, in addition to severe lung infection, can also infect other body sites. Inside the viral envelope, coronavirus possesses a nucleocapsid (N) protein, which is very important for its replication, antigenesis, pathogenesis, virulence, infection and, dissemination¹⁴. This N protein is helically symmetrical with RNA coiling around it¹⁵. All heredity information for viral spike protein synthesis, replication mechanism, other viral encoded protein synthesis, host cell docking and damage, and disease progression are encoded by the viral RNA in N protein^16,17. Coronavirus N protein determines all aspects of viral pathogenesis. In our study, we attempted to characterize the N protein. The N proteins present in other coronaviruses were also compared during this study. We analyzed the molecular composition and amino acid sequence of N present in SARS-CoV-2 and other related viruses like SARS-CoV-1, bat-CoV, and Middle East respiratory syndrome- coronavirus (MERS-CoV). This will help virologists to study viral replication, viral-host immunopathogenesis, and vaccine development to combat the current COVID-19 pandemic. To date, the COVID-19 pandemic has resulted in more than 3,672,238 cases and 254,045 deaths worldwide (WHO-Situation Report-108). This figure is rapidly increasing worldwide, suggesting that SARS-CoV-2 is rapidly disseminating among communities. The rapid infection rate may be attributed to the low virus infectious dose required to elicit infection (0.01 PFU/ml) in an individual¹⁸. Reports suggest that temperature variation in different countries also plays an important role in the spread of COVID-19^19,20. Other studies speculate that individuals already immunized with the BCG vaccine have protection against this disease^21,22; however, there is no effective vaccine available for COVID-19. There are also no antiviral drugs available for COVID-19. Some scientists are empirically employing antimalarial drugs like hydroxychloroquine, and antibiotics such as azithromycin, for COVID-19^23–25. The USA Food and Drug Administration (FDA) permitted the use of the antiviral drug remdesivir for COVID-19 patients. The actual antiviral effects of these drugs against COVID-19 have not been thoroughly proven²⁶. In these circumstances, there is a need for a suitable killed/attenuated vaccine, which combats SARS-CoV-2, for immunoprophylaxis. If an effective COVID-19 vaccine is established, it will elicit a good immune response containing SARS-CoV-2 infection in the community after administration. To develop a vaccine and antiviral drugs against COVID-19, N protein molecular characterization is crucial. Therefore, in this study, we employed various bioinformatics tools, GenBank, and the European nucleotide archive (ENA) to compare SARS-CoV-2 N protein with other related coronaviruses. This study emphasizes and suggests ways to contain and combat COVID-19.