Electrochemiluminescence resonance energy transfer biosensing platform between g-C₃N₄ nanosheet and Ru–SiO₂@FA for dual-wavelength ratiometric detection of SARS-CoV-2 RdRp gene

Abstract

Rational detection of syndrome coronavirus 2 (SARS-CoV-2) is crucial to prevention, control, and treatment of disease. Herein, a dual-wavelength ratiometric electrochemiluminescence (ECL) biosensor based on resonance energy transfer (RET) between g-C₃N₄ nanosheets and Ru–SiO₂@folic acid (FA) nanomaterials was designed to realize ultrasensitive detection of SARS-CoV-2 virus (RdRp gene). Firstly, the unique g-C₃N₄ nanosheets displayed very intense and stable ECL at 460 nm, then the triple helix DNA was stably and vertically bound to g-C₃N₄ on electrode by high binding affinity between ssDNA and g-C₃N₄. Meanwhile, trace amounts of target genes were converted to a large number of output by three-dimensional (3D) DNA walker multiple amplification, and the output bridged a multifunctional probe Ru–SiO₂@FA to electrode. Ru–SiO₂@FA not only showed high ECL at 620 nm, but also effectively quenched g-C₃N₄ ECL. As a result, ECL decreased at 460 nm and increased at 620 nm, which was used to design a rational ECL biosensor for detection of SARS gene. The results show that the biosensor has excellent detection sensitivity for RdRp gene with a dynamic detection range of 1 fM to 10 nM and a limit of detection (LOD) of 0.18 fM. The dual-wavelength ratio ECL biosensor has inestimable value and application prospects in the fields of biosensing and clinical diagnosis.