Cationic Surfactants as Disinfectants against SARS-CoV-2
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of the Surfactant Structure on the Virucidal Efficacy
2.2. Concentration Dependence of Virucidal Activity of the Most Efficient Surfactants
2.3. Time Dependence of Virucidal Activity of the Most Efficient Surfactants
3. Materials and Methods
3.1. Surfactants
3.2. Cells
3.3. Virus and Virus Titration
3.4. Fluorescence Spectroscopy
3.5. Evaluation of Virucidal Activity
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Surfactant | Abbreviation | Hydrophobic Tail | Critical Micelle Concentration (cmc), mM | Hydrophile-Lipophile Balance (HLB) | Virus Titer | Inhibition Coefficient IC, % | ||
---|---|---|---|---|---|---|---|---|
Control Ac | Experiment Ae | Log10 Reduction A | ||||||
Benzyldimethyldode-cylammonium chloride | C12BAC | C12 | 1.2 * | 14.2 ** | 7.00 | 0.00 | 7.00 | 100 |
Benzalkonium chloride | BAC | C12 (70%), C14 (30%) | 0.37 *, 0.43 [19] | 13.9 ** | 7.00 | 0.00 | 7.00 | 100 |
Didodecyldimethyl-ammonium bromide | C12-C12DMA | C12-C12 | 0.08 [20], 0.15 [21] | 10 [22] | 7.00 | 0.00 | 7.00 | 100 |
Dodecyltrimethyl-ammonium chloride | C12DMA | C12 | 21.3 [23] | 15 [22], 17.1 [24], 18.5 [25] | 7.00 | 5.75 | 1.25 | 18 |
Cetylpyridinium chloride | C16Py | C16 | 0.9 [26,27,28] | 14.5 ** | 7.00 | 0.00 | 7.00 | 100 |
Dodecylpyridinium chloride | C12Py | C12 | 15 [29] | 16.4 [30] | 7.00 | 4.75 | 2.25 | 32 |
Octylpyridinium bromide | C8Py | C8 | 190 [29] | 18.3 ** | 6.50 | 6.25 | 0.25 | 3.8 |
Surfactant | Concentration | Virus Titer | Inhibition Coefficient IC, % | |||
---|---|---|---|---|---|---|
mM | wt% | Control Ac | Experiment Ae | Log10 Reduction A | ||
Benzyldimethyldodecylammo- nium chloride (C12BAC) | 0.0224 | 0.0008 | 6.5 | 5.5 | 1.0 | 15 |
0.112 | 0.0038 | 6.5 | 2.0 | 4.5 | 69 | |
0.56 | 0.0190 | 6.5 | 0 | 6.5 | 100 | |
2.8 | 0.0950 | 6.5 | 0 | 6.5 | 100 | |
50% effective concentration (EC50) 0.081 mM | ||||||
Benzalkonium chloride (BAC) | 0.0048 | 0.0002 | 6.5 | 6.0 | 0.5 | 7.7 |
0.0224 | 0.0008 | 6.5 | 5.5 | 1.0 | 15 | |
0.112 | 0.0039 | 6.5 | 0 | 6.5 | 100 | |
0.56 | 0.0195 | 6.5 | 0 | 6.5 | 100 | |
2.8 | 0.0970 | 6.5 | 0 | 6.5 | 100 | |
50% effective concentration (EC50) 0.072 mM | ||||||
Didodecyldimethylammonium bromide (C12-C12DMA) | 0.0048 | 0.0002 | 6.75 | 6.5 | 0.25 | 3.7 |
0.0224 | 0.0010 | 6.75 | 2.0 | 4.75 | 70 | |
0.112 | 0.0052 | 6.75 | 0 | 6.75 | 100 | |
0.56 | 0.0259 | 6.75 | 0 | 6.75 | 100 | |
2.8 | 0.1295 | 6.75 | 0 | 6.75 | 100 | |
50% effective concentration (EC50) 0.016 mM | ||||||
Cetylpyridinium chloride (C16Py) | 0.0048 | 0.0002 | 6.75 | 6.5 | 0.25 | 3.7 |
0.0224 | 0.0008 | 6.75 | 6.0 | 0.75 | 11 | |
0.112 | 0.0038 | 6.75 | 0 | 6.75 | 100 | |
0.56 | 0.0190 | 6.75 | 0 | 6.75 | 100 | |
2.8 | 0.0950 | 6.75 | 0 | 6.75 | 100 | |
50% effective concentration (EC50) 0.101 mM |
Surfactant | Contact Time | Virus Titer | Inhibition Coefficient IC, % | ||
---|---|---|---|---|---|
Control Ac | Experiment Ae | Log10 Reduction A | |||
Benzalkonium chloride (BAC) | 5 s | 7.50 | 0.00 | 7.50 | 100 |
15 s | 7.50 | 0.00 | 7.50 | 100 | |
30 s | 7.50 | 0.00 | 7.50 | 100 | |
5 min | 7.50 | 0.00 | 7.50 | 100 | |
Didodecyldimethylammonium bromide (C12-C12DMA) | 5 s | 8.00 | 0.00 | 8.00 | 100 |
15 s | 8.00 | 0.00 | 8.00 | 100 | |
30 s | 8.00 | 0.00 | 8.00 | 100 | |
5 min | 8.00 | 0.00 | 8.00 | 100 | |
Cetylpyridinium chloride (C16Py) | 5 s | 7.75 | 6.00 | 1.75 | 23 |
15 s | 7.75 | 4.50 | 3.25 | 42 | |
30 s | 7.75 | 3.25 | 4.25 | 55 | |
5 min | 7.75 | 0.00 | 7.75 | 100 |
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Karamov, E.V.; Larichev, V.F.; Kornilaeva, G.V.; Fedyakina, I.T.; Turgiev, A.S.; Shibaev, A.V.; Molchanov, V.S.; Philippova, O.E.; Khokhlov, A.R. Cationic Surfactants as Disinfectants against SARS-CoV-2. Int. J. Mol. Sci. 2022, 23, 6645. https://doi.org/10.3390/ijms23126645
Karamov EV, Larichev VF, Kornilaeva GV, Fedyakina IT, Turgiev AS, Shibaev AV, Molchanov VS, Philippova OE, Khokhlov AR. Cationic Surfactants as Disinfectants against SARS-CoV-2. International Journal of Molecular Sciences. 2022; 23(12):6645. https://doi.org/10.3390/ijms23126645
Chicago/Turabian StyleKaramov, Eduard V., Viktor F. Larichev, Galina V. Kornilaeva, Irina T. Fedyakina, Ali S. Turgiev, Andrey V. Shibaev, Vyacheslav S. Molchanov, Olga E. Philippova, and Alexei R. Khokhlov. 2022. "Cationic Surfactants as Disinfectants against SARS-CoV-2" International Journal of Molecular Sciences 23, no. 12: 6645. https://doi.org/10.3390/ijms23126645