qRT-PCR Platforms for Diagnosing and Reporting SARS-CoV-2 Infection in Human Samples

Highlights

• Single- and multiplex qRT-PCR protocols for detecting SARS-CoV-2 viral RNA

• FDA EUA certified ready-to-use protocols for use in CLIA-certified laboratories

• Protocols utilize reagents commonplace to basic science research laboratories

• Included R script allows for automated interpretations of results

Summary

The protocols herein outline the use of qRT-PCR to detect the presence of SARS-CoV-2 genomic RNA in patient samples. In order to cope with potential fluctuations in supply chain and testing demands and to enable expedient adaptation of reagents and assays on hand, we include details for three parallel methodologies (one- and two-step singleplex and one-step multiplex assays). The diagnostic platforms described can be easily adapted by basic science research laboratories for SARS-CoV-2 diagnostic testing with relatively short turnaround time.

For complete details on the use and execution of this protocol, please refer to Vanuytsel et al. (2020).