Dual Ligand-Induced Photoelectrochemical Sensing by Integrating Pt/MoS2 Heterostructure and AU Polyhedra for Sensitive Detection of SARS-CoV-2

17 Pages Posted: 22 Sep 2022

See all articles by Haolin Li

Haolin Li

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety

Jialin Zhao

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety

Ting Wu

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety

Zhao Fu

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety

Wei Zhang

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety

Zheng Lian

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety

Shuangfei Cai

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety

Rong Yang

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety

Abstract

The continuous evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with discovery of multiple mutants, has caused widespread panic and concern worldwide. The rapid antigen detection method via a single ligand recognition, although currently implemented in many countries, remains challenging for mutated antigens. Herein, we present a novel strategy using a dual recognition by two types of targeted ligands, based on photoelectrochemical (PEC) sensing for detection of SARS-CoV-2 spike protein. To demonstrate this strategy, the specific antibodies are modified onto the photoactive material with a supported nanostructure, created by loading the Pt nanoparticles onto MoS2 nanosheets (Pt/MoS2) to boost photon-to-electricity conversion efficiency. By subsequent binding of the targeted aptamers to the Au polyhedra, which act as a signal amplifier to suppress PEC photocurrent by competing with the Pt/MoS2 for the absorption of excitation light energy, the dual recognition is successfully achieved. The constructed biosensor not only shows satisfactory stability, and high sensitivity, but is effective for test of the pseudovirus of SARS-CoV-2. The work provides useful advance for the development of PEC biosensors for sensitive detection of SARS-CoV-2.

Note: br>
Funding Information: This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB36000000), and the National Key R&D Program of China (2021YFE0112600; 2017YFA0303400). NSFC-RGC (11861161002) and National Natural Science Foundation of China (12174032).

Declaration of Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Keywords: photoelectrochemical sensing, dual ligand, MoS2, SARS-CoV-2, spike protein

Suggested Citation

Li, Haolin and Zhao, Jialin and Wu, Ting and Fu, Zhao and Zhang, Wei and Lian, Zheng and Cai, Shuangfei and Yang, Rong, Dual Ligand-Induced Photoelectrochemical Sensing by Integrating Pt/MoS2 Heterostructure and AU Polyhedra for Sensitive Detection of SARS-CoV-2. Available at SSRN: https://ssrn.com/abstract=4206901 or http://dx.doi.org/10.2139/ssrn.4206901

Haolin Li

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety ( email )

Jialin Zhao

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety ( email )

Ting Wu

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety ( email )

Zhao Fu

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety ( email )

Wei Zhang

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety ( email )

Zheng Lian

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety ( email )

Shuangfei Cai (Contact Author)

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety ( email )

Rong Yang

National Center for Nanoscience and Technology - CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety ( email )

Do you have negative results from your research you’d like to share?

Paper statistics

Downloads
20
Abstract Views
143
PlumX Metrics