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Laboratory Detection of SARS-CoV-2: A Review of the Current Literature and Future Perspectives

22 Pages Posted: 1 Mar 2022 Publication Status: Published

See all articles by Xiao-Dong Mao

Xiao-Dong Mao

affiliation not provided to SSRN

Kang-Sheng Liu

affiliation not provided to SSRN

Tai-Ping Li

Nanjing Medical University - Affiliated Brain Hospital

Abstract

Coronavirus disease 2019 (COVID-19) is now a major public health threat worldwide. The infectivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is extremely strong. Since the major target of the virus is the lung, the infection can lead to the respiratory distress syndrome, multiple organ failure, and even death. The studies on viral structure and infection mechanism have found that angiotensin-converting enzyme 2 (ACE2), a key enzyme in the RAS system, is the receptor of SARS-CoV-2, and SARS-CoV-2 affects the target via ACE2. Currently, the detection of SARSCoV-2 is mainly achieved by using open plate real-time reverse-transcription polymerase chain reaction (RT-PCR). But open plate method has some limitations, such as a high false-negative rate, cumbersome manual operation, aerosol pollution and leakage risks. Therefore, a convenient method to detect SARS-CoV-2 is urgently needed for rapid test and timely control of the epidemic when resources are limited. In this paper, the existing real-time detection methods for novel coronavirus and their principles are summarized, in order to provide a reference for real-time screening of coronavirus in areas with insufficient detection capacity and inadequate medical resources. The establishment of a rapid, simple, sensitive and specific method for SARS-CoV-2 detection is of vital importance, especially in the flu season, so that SARS-CoV-2 can be differentially diagnosed, and effective measures can be taken.

Funding Information: Funding was not availed for this article,

Conflict of Interests: All authors declare they have no actual or potential competing interests.

Keywords: Novel coronavirus, SARS-CoV-2, gene sequencing, Nucleic acid testing, Gene editing techniques

Suggested Citation

Mao, Xiao-Dong and Liu, Kang-Sheng and Li, Tai-Ping, Laboratory Detection of SARS-CoV-2: A Review of the Current Literature and Future Perspectives. Available at SSRN: https://ssrn.com/abstract=4047093 or http://dx.doi.org/10.2139/ssrn.4047093

Xiao-Dong Mao

affiliation not provided to SSRN ( email )

No Address Available

Kang-Sheng Liu

affiliation not provided to SSRN ( email )

No Address Available

Tai-Ping Li (Contact Author)

Nanjing Medical University - Affiliated Brain Hospital ( email )

300 Guangzhou Road
Nanjing, Jiangsu 210029
China

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