Issue 6, 2022
From the journal:

Nanoscale Advances

Site-specific recognition of SARS-CoV-2 nsp1 protein with a tailored titanium dioxide nanoparticle – elucidation of the complex structure using NMR data and theoretical calculation†

Peter Agback,*a   Tatiana Agback, ORCID logo a   Francisco Dominguez,b   Elena I. Frolova,b   Gulaim A. Seisenbaeva ORCID logo a  and  Vadim G. Kessler ORCID logo *a  

* Corresponding authors

a Department of Molecular Sciences, Swedish University of Agricultural Sciences, Box 7015, Uppsala, Sweden
E-mail: peter.agback@slu.se, vadim.kessler@slu.se

b Department of Microbiology, University of Alabama at Birmingham, 1720 2nd Ave South Birmingham, USA

Abstract

The ongoing world-wide Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) pandemic shows the need for new potential sensing and therapeutic means against the CoV viruses. The SARS-CoV-2 nsp1 protein is important, both for replication and pathogenesis, making it an attractive target for intervention. In this study we investigated the interaction of this protein with two types of titania nanoparticles by NMR and discovered that while lactate capped particles essentially did not interact with the protein chain, the aminoalcohol-capped ones showed strong complexation with a distinct part of an ordered α-helix fragment. The structure of the forming complex was elucidated based on NMR data and theoretical calculation. To the best of our knowledge, this is the first time that a tailored titanium oxide nanoparticle was shown to interact specifically with a unique site of the full-length SARS-CoV-2 nsp1 protein, possibly interfering with its functionality.

Graphical abstract: Site-specific recognition of SARS-CoV-2 nsp1 protein with a tailored titanium dioxide nanoparticle – elucidation of the complex structure using NMR data and theoretical calculation

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Article information

DOI
https://doi.org/10.1039/D1NA00855B
Article type
Communication
Submitted
07 Dec 2021
Accepted
16 Feb 2022
First published
17 Feb 2022
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2022,4, 1527-1532

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Site-specific recognition of SARS-CoV-2 nsp1 protein with a tailored titanium dioxide nanoparticle – elucidation of the complex structure using NMR data and theoretical calculation

P. Agback, T. Agback, F. Dominguez, E. I. Frolova, G. A. Seisenbaeva and V. G. Kessler, Nanoscale Adv., 2022, 4, 1527 DOI: 10.1039/D1NA00855B

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