Development of an assay to rigorously assess cross-well contamination inherent to open liquid handling instruments.
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Clinical assessment of generic magnetic bead-based extraction protocols for SARS-CoV-2 nucleic acid testing.
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Development of a standard operating procedure for clinical SARS-CoV-2 testing on an open liquid handling platform.
Abstract
The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized to minimize cross-well contamination while maintaining sensitivity.
