VIR-CRISPR: Visual in-one-tube ultrafast RT-PCR and CRISPR method for instant SARS-CoV-2 detection
Graphical abstract
We proposed an ultrasensitive, instant and visual detection method termed as VIR-CRISPR for point-of-care SARS-CoV-2 detection. It integrated ultrafast RT-PCR and CRISPR in-one-tube with high specificity and detection sensitivity at nearly single molecule level, holding great promise for rapid clinical diagnosis.
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(1) Rui Wang, Ph.D., is a research scientist in Human Phenome Institute of Fudan University. Her research is focused on rapid nucleic acid amplification and detection, microfluidics and nanotechnology.
(2) Yongfang Li, Ph.D. candidate at the department of Chemistry in Fudan University. Her current research interests are focused on the luminescent materials and their applications in in-vitro diagnosis.
(3) Yanan Pang received his Doctor's degree from China Second Military Medical University in 2019. Now, he is working as assistant research fellow in Changhai Hospital, Shanghai, China. He is major in non-coding RNA and the early diagnosis of cancer.
(4) Shihua Luo, Ph.D., is a a doctor at Department of Traumatology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University. He is mainly focus on disease treatment.
(5) Chunyan Qian, MD, the director of the clinical laboratory, Linping Campus, The Second Affiliated Hospital of Zhejiang University School of Medicine, focusing on the diagnosis of infectious diseases.