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Delivery of Experimental mRNA Vaccine Encoding the RBD of SARS-CoV-2 by Jet Injection

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Bulletin of Experimental Biology and Medicine Aims and scope

We studied a needle-free jet injection delivery of an experimental mRNA vaccine encoding the receptor-binding domain of the SARS-CoV-2 S protein (mRNA-RBD). Immunization of BALB/c mice with mRNA-RBD by a needle-free jet injector induced high levels of antibodies with virus-neutralizing activity and a virus-specific T-cell response. The immune response was low in the group of mice that received intramuscular injection of mRNA-RBD. The effectiveness of this simple and safe method of mRNA delivering has been demonstrated. Thus, jet injection of mRNA vaccine can be a good alternative to lipid nanoparticles.

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Authors and Affiliations

  1. State Research Center of Virology and Biotechnology “VECTOR”, Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Koltsovo, Novosibirsk region, Russia

    D. N. Kisakov, L. A. Kisakova, S. V. Sharabrin, V. A. Yakovlev, E. V. Tigeeva, M. B. Borgoyakova, E. V. Starostina, A. V. Zaikovskaya, A. P. Rudometov, N. B. Rudometova, L. I. Karpenko & A. A. Ilyichev

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  1. D. N. Kisakov
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  2. L. A. Kisakova
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  3. S. V. Sharabrin
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  4. V. A. Yakovlev
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  5. E. V. Tigeeva
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  6. M. B. Borgoyakova
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  7. E. V. Starostina
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  8. A. V. Zaikovskaya
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  9. A. P. Rudometov
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  10. N. B. Rudometova
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  11. L. I. Karpenko
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  12. A. A. Ilyichev
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Corresponding author

Correspondence to D. N. Kisakov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 176, No. 12, pp. 751-756, December, 2023

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Kisakov, D.N., Kisakova, L.A., Sharabrin, S.V. et al. Delivery of Experimental mRNA Vaccine Encoding the RBD of SARS-CoV-2 by Jet Injection. Bull Exp Biol Med 176, 776–780 (2024). https://doi.org/10.1007/s10517-024-06107-x

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  • DOI: https://doi.org/10.1007/s10517-024-06107-x

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